Insulin radioimmunoassay by the charcoal-dextran technique
نویسندگان
چکیده
منابع مشابه
Laboratory application of the dextran-coated-charcoal radioimmunoassay of insulin.
A modification of the method for radioimmunoassayof insulin is described, which achievessatisfactory sensitivity while employing commercially available reagents and antiserums. These modifications consist mainly in lowering the initial percentage binding, reducing the amount of labeled insulin, and preincubating the unlabeled hormone with antiserum. Application of the linear isotope dilution fo...
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Biopsy specimens from 21 women with primary operable breast cancer were analyzed in a double blind study designed to compare fluorescent antibody methods for detection of estrogen receptors with estrogen receptors measured biochemically with a previously reported dextran coated charcoal technique (DCC). Fluorescent antibody methoids employed both direct techniques: (1) rabbit antisera to 6-O-Ca...
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Charcoal adsorption of unbound acyclovir rather than ammonium sulfate precipitation of bound acyclovir to facilitate the separation of bound antigen from free antigen gave rise to a radioimmunoassay which was quicker yet still as sensitive and accurate as that previously used.
متن کاملPreparation of ligand-free human serum for radioimmunoassay by adsorption on activated charcoal.
I describe a convenient method for stripping hormones and other ligands from human serum. Formation of a serum/charcoal colloid is avoided by incubating serum on a limited surface area with activated charcoal, in a lateral oscillating shaker. Particle-free serum of negligible turbidity is obtained without ultracentrifugation by ejecting the centrifugate through a cellulose ester membrane. By th...
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ژورنال
عنوان ژورنال: Diabetologia
سال: 1972
ISSN: 0012-186X,1432-0428
DOI: 10.1007/bf01225574